Advanced Training Course in Cell and Molecular Techniques

 3 -15 february 2018

 

Institute of Biotechnology

Ferdowsi University of Mashhad

An advanced training course in Cell and Molecular Techniques
will be presented for international postgraduate participants.
This two-week course provides hands-on introduction to
useful biology tools in cell and molecular levels. 


 

 

TOPICS:

  •  Stem cell derivation from rat bone marrow
  •  Cell cycle analysis by flow cytometry
  •  DNA isolation, conventional PCR and electrophoresis
  •  Reverse transcription and real time PCR
  •  Prokaryotic gene cloning
 

APPLICATION & DEADLINES:

In total, 15 participants will be selected based on qualifications. 
Please send your CV and a short motivation description in one
pdf file via email to این آدرس ایمیل توسط spambots حفاظت می شود. برای دیدن شما نیاز به جاوا اسکریپت دارید  


 

 

Institute of Biotechnology
Ferdowsi University of Mashhad
Mashhad, Iran
Website: fib.um.ac.ir
Email: این آدرس ایمیل توسط spambots حفاظت می شود. برای دیدن شما نیاز به جاوا اسکریپت دارید
Coordinator:
Dr. Fatemeh B. Rassouli
Email: این آدرس ایمیل توسط spambots حفاظت می شود. برای دیدن شما نیاز به جاوا اسکریپت دارید

Application deadline:   20 DECEMBER 2017

Selection of participants:   3 JANUARY 2018

 

 

Agenda


 

Introduction to biology techniques
Overview of workshops (cell culture, stem cell derivation, cell cycle analysis,
  DNA and protein isolation, PCR, electrophoresis, reverse transcription,
  RT-PCR, immunocytochemistry, prokaryotic gene cloning)
Introduction to cell culture
Practice on cell culture


 

Day 1
Introduction to stem cells 
Practice on stem cell derivation from rat bone marrow
 Day 2
Introduction to cell growth and apoptosis 
Practice on cell cycle analysis by flow cytometry
 Day 3
Principles of DNA and protein isolation, PCR and electrophoresis 
Practice on DNA and protein isolation from cells, PCR and DNA and
  protein electrophoresis
 Day 4 & 5
Introduction to gene expression analysis at mRNA and protein levels;
  reverse transcription and immunostaining
Practice on RNA isolation, cDNA synthesis and RT-PCR
Practice on immunocytochemistry
 Day 6, 7 & 8
Introduction to genetic engineering
Practice on prokaryotic gene cloning
 Day 9, 10 & 11
Visiting laboratories in Faculty of Science, Faculty of Veterinary Medicine, and
  Faculty of Agriculture (Ferdowsi University of Mashhad), and ACECR, Mashhad
Group meeting
 Day 12